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2190 次微環(huán)境多靶標(biāo)蛋白分析系統(tǒng)升級前后功能對比 2022-10-21
深入分析組織微環(huán)境對于理解疾病生物學(xué)及其進(jìn)展至關(guān)重要。過去幾年，芯片流式技術(shù)ChipCytometry產(chǎn)品在組織免疫分析中展現(xiàn)出的巨大應(yīng)用價值，已經(jīng)得到了世界各地用戶的廣泛認(rèn)可和肯定，這種先進(jìn)的
16234 次 CellSens standard軟件主要功能操作說明 2018-5-22
一、明場實時圖像調(diào)節(jié)及拍照保存1.打開cellSens軟件后，首先在攝像控制工具窗口中切換相機(jī)芯片為“彩色模式”，此時相機(jī)自動切換為彩色芯片進(jìn)行明場圖像采圖；接下來點擊“實時觀
2654 次脾臟、胸腺和淋巴結(jié)中單個核細(xì)胞分離和培養(yǎng) 2017-3-16
基本方案從小鼠脾臟、胸腺和淋巴結(jié)制備單個核細(xì)胞分離和培養(yǎng)。實驗材料1.RPMI-5或DMEM-5完全培養(yǎng)基。2.新鮮分離的小鼠器官：≤6周齡的小鼠胸腺；6周至6個月齡的小鼠脾臟和淋巴結(jié)。3.60mm&time
2398 次 3H-Thymidine Uptake by Cultured Cells 2012-6-20
Materials Fibroblast cells in log phase growthCa , Mg free-phosphate buffered saline (PBSA)5% (w/v) Glutaraldehyde (GTA)2% (w/v) Perchloric Acid (PCA)Subbed slides (coated with chr
3270 次 Culture of human venous smooth muscle cells 2012-5-23
Culture of human venous smooth muscle cells1.Explants of the 2 groups of skins (control subjects and patients with varicose veins) were prepared according to the method described f
3088 次 Isolation of human prostatic smooth muscle cells 2012-5-8
Isolation of human prostatic smooth muscle cells Human prostate tissues1.Human hyperplastic prostates were obtained during surgery from four men through transurethral resection of
2837 次 Isolation of human primary gastric mucosa epithelial cells 2012-4-19
Isolation of human primary gastric mucosa epithelial cells1.A piece of gastric mucosa (∼3 cm2) was obtained from the normal appearing mucosa of the stomach at surgery. 2.The
1805 次 Isolation of papillary cells 2012-3-26
Isolation of papillary cellsIsolation of renal papillary cells1.For isolation of papillary cells, kidneys were harvested and kept in HBSS containing 15 mM HEPES, penicillin/strept
5636 次 Isolation of peripheral blood mononuclear cells (PBMC) 2012-1-16
Isolation of peripheral blood mononuclear cells (PBMC) 1.Acid-citrate dextrose-treated blood (450 ml) was obtained from donors. 2.PBMC were isolated by centrifugation over lymphoc
3242 次 Stem cell characteristics of amniotic epithelial (AE) cells 2011-12-24
Stem cell characteristics of amniotic epithelial (AE) cells Isolation of AE Cells1.Human placentae were obtained with the approval of the institutional review board, after uncompli
3914 次 Isolation of human primary ovarian surface epithelial cells 2011-12-12
Isolation of human primary ovarian surface epithelial cells1.Surface epithelial cells from normal ovaries from surgical residual specimen were isolated using standard and Institut
1638 次 Isolation of human prostatic epithelial cells 2011-11-25
Isolation of human prostatic epithelial cells1.A small piece of tissue from each specimen was removed and minced. 2.The tissue was digested with collagenase overnight. 3.To remove
3328 次 Isolation of stromal vascular cells from human adipose tissue 2011-9-30
Isolation of stromal vascular cells from human adipose tissue Stromal stem cells proliferate in vitro and may be differentiated along several lineages. The scarcity of stromal ste
3400 次 Isolation and cultivation of endothelial progenitor cells (EPCs) 2011-9-30
Isolation and cultivation of endothelial progenitor cells (EPCs) Circulating bone marrow (BM)–derived endothelial progenitor cells (EPCs) are recruited to the site of tissue rege
2334 次 Isolation of rodent pancreatic β cells 2011-8-1
Isolation of rodent pancreatic β cells 1.Adult rats weighing 250-350g were anesthetized, sacrificed and immediately used for pancreas sampling.2.Rat islets were isolated from mal
2384 次 Culture of human renal proximal tubule cells and renal cortical fibroblasts 2011-7-11
Culture of human renal proximal tubule cells and renal cortical fibroblasts 1.The method for primary culture of human renal proximal tubule cells (PTC) and renal cortical fibrobla
2137 次 Isolation of human colonic epithelial cells (EC) 2011-6-21
Isolation of human colonic epithelial cells (EC)1.Human colonic epithelial cells (EC) were isolated from freshly resected colonic surgical specimens obtained. 2.All diagnoses were
2460 次 Isolation and Culture of Human Liver Stem Cells 2011-4-1
Isolation and Culture of Human Liver Stem CellsMaterials and Methods1.Human hepatocytes were isolated from fresh surgical specimens of patients undergoing hepatectomies. Healthy li
2200 次 FITC標(biāo)記抗體-Marsshall氏法 2010-10-13
FITC標(biāo)記抗體-Marsshall氏法材料：抗體球蛋白溶液、0.5mol/L pH9.0碳酸鹽緩沖液、無菌生理鹽水、異硫氰酸熒光素、1%硫柳汞水溶液、三角燒瓶（25—50ml）、冰及冰槽（或1000ml燒杯）、電磁攪拌器
3055 次原代細(xì)胞骨架的染色方法 2010-10-11
原代細(xì)胞骨架的染色方法微絲的顯示方法步驟：1. 用PBS液漂洗蓋片培養(yǎng)的原代細(xì)胞3次，每次30s；2. 用2%的甲醛/PBS液固定原代細(xì)胞3min；3. 用0.5%的三硝基甲苯/PBS處理3次，每次10min；4. PBS漂洗