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Application of SMALP in Screening of Different Membrane Proteins

瀏覽次數:593 發布日期:2024-11-19  來源:本站 僅供參考,謝絕轉載,否則責任自負
Description
The SMALP, among all synthetic nanodisc products has been developed for for the solubilization of membrane proteins from all kinds of cell membranes and subsequent isolation & purification of said membrane proteins. After dissolving SMALP 200 + buffer powder in dH2O, the product is ready-to-use. SMALP 200 has a yellowish color in solution. After successfull solubilization of the membrane protein, the protein can be purified using affinity chromatography. For membrane protein purification we recommed to use the Rho1D4-tag. Cube Biotech offers the matching products for this purpose.
Datasheets
FEATURE  
State Lyophilized Powder, to be solved with water
Polymer styrene-to-maleic anhydride ratio 2:1
Molecular weight 6,500 Da
pH after solving 7.5
Solubility 40 %
Divalent cationic tolerance < 5 mM
Color Yellowish
Odor Odorless
Freeze-thaw stable? Yes
Shipping Temperature ambient temperature
Storage of lyophilized copolymer -20°C for several years
Storage of dissolved copolymer 2-8°C for several days
Other noteworthy feature Most commonly used SMA
Structure  
 
Citations
STABILIZED PROTEIN YEAR AUTHOR
Bacterial Membrane Proteins 2022 Noh I., Guo Z., Zhou J., Gao W., Fang R.H., Zhang L.
- 2023 Hernandez L.M.R. & Levental I.
Alpha-Toxin degrading proteins 2023 Sun L., Wang D., Noh R., Fang H., Gao W., Zhang L.
ATP-binding cassette (ABC) transporter 2023 Dimitrova V.A., Song S., Karagiaridi A., Marand A., Pinkett H.W.
Env-protein of HIV 2023 Zhou R., Zhang S., Nguyen H.T., Ding H., Gaffney A., Kappes J.C., Smith III. A.B.S, Sodroski J.G.
 
Lab Results
SMALP Screening: Different membrane protein usually perform different under different SMAs variants respectively. To ease the resulting screening process we compiled all of our SMAs into a convenient SMALP Screening Set. Furthermore if you like to compare proteins's performance between SMA and DIBMA in synthetic nanodiscs, we also offer our, that includes both our SMA and DIBMA products.
 
Figure 1: Western Blot of a purified membrane protein after stabilization through SMA. Reproduced with permission from Ms S. Nestorow, University of Birmingham.
 
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