如何使用器官芯片技術(shù)構(gòu)建更真實(shí)的體外環(huán)境？

器官芯片（Organ-on-a-Chip）技術(shù)是一種新型的藥物檢測技術(shù)。該技術(shù)通過結(jié)合微流控技術(shù)提高傳統(tǒng)細(xì)胞培養(yǎng)的生理相關(guān)性。芯片上的器官是3D組織，可以更好地體現(xiàn)出體內(nèi)組織的復(fù)雜性，包括3D形態(tài)、細(xì)胞外基質(zhì)嵌入、多種細(xì)胞類型、血管結(jié)構(gòu)和灌流。

In this presentation I will introduce culture and interrogation of complex 3D tissues, such as liver, kidney, gut and brain tissue. The tissues are grown on the OrganoPlate® platform, that allow culturing of over 40 tissues in parallel. The tissues are embedded in an extracellular matrix gel and comprise both stromal tissue, bloodvessel structures as well as epithelial barriers with clear apical and basal sides. The platform can be interrogated using automated imaging equipment as well as all standard equipment including fluorescence-based assays, immunohistochemical staining, barrier integrity monitoring, transport studies, viability assays, qRT-PCR, ELISA’s and many others. Last but not least, operation of the platform is thus straightforward that an end-user who is not necessarily an expert in the field of microfluidics is able to perform the study. In this presentation I will show examples of Organ-on-a-Chip tissues for use in drug safety testing and disease modelling.

此部分中，來自Molecular Devices公司的高級產(chǎn)品經(jīng)理艾平將帶來的關(guān)于“探索生命科學(xué)奧秘的高內(nèi)涵成像解決方案”的介紹。重點(diǎn)介紹高內(nèi)涵概念、在基于細(xì)胞成像的生命科學(xué)研究以及藥物篩選和毒性安全評價(jià)中的應(yīng)用、以及Molecular Devices提供的高內(nèi)涵成像分析解決方案。